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The Dutch giants saw off Young Boys in the last round to reach the quarter-finals and haven’t lost a competitive game since December 9.
Roma meanwhile beat Shakhtar to reach the last eight but have struggled on the road this term, losing four of their last 10 away matches.
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Roma will be without Stephan El Shaawrawy as he has a thigh injury to recover from
Marash Kumbulla is has a problem with the meniscus in his knee and could miss the rest of the season for Roma.
Martin Stekelenburg is struggling with a knee injury and may not start for Ajax, who are also without Daley Blind.
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